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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
10/11/2023 |
Actualizado : |
10/11/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BORGES, M.A.; CURCIO, B.R.; GASTAL, G.D.A.; GHENO, L.; JUNIOR, A.S.V.; CORCINI, C.D.; NOGUEIRA, C.E.W.; AGUIAR, F.L.N.; GASTAL, E.L. |
Afiliación : |
MORGANA A. BORGES, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; BRUNA R. CURCIO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIZA GHENO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; ANTONIO S. VARELA JUNIOR, Department of Comparative Animal Reproduction, Institute of Biological Sciences, Federal University of Rio Grande, RS, Rio Grande, Brazil; CARINE D. CORCINI, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; CARLOS E.W. NOGUEIRA, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; FRANCISCO L.N. AGUIAR, Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraiba, PB, Sousa, Brazil; EDUARDO L. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States. |
Título : |
Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Reproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814 |
ISSN : |
1642-431X |
DOI : |
10.1016/j.repbio.2023.100814 |
Idioma : |
Inglés |
Notas : |
Article history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- |
Contenido : |
ABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn MenosABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more ... Presentar Todo |
Palabras claves : |
Fixative solution; Mare; Ovarian tissue quality; Preantral follicle morphometry. |
Asunto categoría : |
L50 Fisiología y bioquímica animal |
Marc : |
LEADER 03765naa a2200301 a 4500 001 1064365 005 2023-11-10 008 2023 bl uuuu u00u1 u #d 022 $a1642-431X 024 7 $a10.1016/j.repbio.2023.100814$2DOI 100 1 $aBORGES, M.A. 245 $aEthanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- 520 $aABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn 653 $aFixative solution 653 $aMare 653 $aOvarian tissue quality 653 $aPreantral follicle morphometry 700 1 $aCURCIO, B.R. 700 1 $aGASTAL, G.D.A. 700 1 $aGHENO, L. 700 1 $aJUNIOR, A.S.V. 700 1 $aCORCINI, C.D. 700 1 $aNOGUEIRA, C.E.W. 700 1 $aAGUIAR, F.L.N. 700 1 $aGASTAL, E.L. 773 $tReproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
29/03/2021 |
Actualizado : |
29/03/2021 |
Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
Autor : |
INIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA); INIA TREINTA Y TRES; INIA TACUAREMBÓ |
Afiliación : |
ESTACIÓN EXPERIMENTAL DEL ESTE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ESTACIÓN EXPERIMENTAL DEL NORTE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Arroz. Jornadas virtuales. |
Complemento del título : |
Actividades. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Revista INIA Uruguay, 2021, no.64, p.103. |
Serie : |
(Revista INIA; 64). |
ISSN : |
1510-9011 |
Idioma : |
Español |
Notas : |
Día de campo de arroz zona centro norte (4 febrero 2021); Día de campo de la UEPL (4 marzo 2021). |
Contenido : |
Como ya es tradicional en el mes de febrero, se realizó el día de campo de arroz para la zona norte.
En este día de campo virtual se presentaron avances en dos líneas de investigación: mejoramiento genético y manejo del cultivo en relación a resistencia a herbicidas
y manejo del riego. Al igual que en años anteriores, se presentaron resultados de la Red Nacional de Evaluación de cultivares INIA - INASE.
El 4 de marzo tuvo lugar el Dia de campo de arroz para la zafra 20-21 correspondiente a la Unidad Experimental Paso de la Laguna. El objetivo de esta actividad fue que los participantes adquieran un panorama de los trabajos de investigación que INIA viene desarrollando, permitiendo integrar esta información con lo que se está haciendo a nivel productivo y generar así nuevos elementos para analizar los resultados de la investigación. |
Thesagro : |
ARROZ. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15426/1/Revista-INIA-64-Marzo-2021-p-103.pdf
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Marc : |
LEADER 01483naa a2200193 a 4500 001 1061904 005 2021-03-29 008 2021 bl uuuu u00u1 u #d 022 $a1510-9011 100 1 $aINIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA) 245 $aArroz. Jornadas virtuales.$h[electronic resource] 260 $c2021 490 $a(Revista INIA; 64). 500 $aDía de campo de arroz zona centro norte (4 febrero 2021); Día de campo de la UEPL (4 marzo 2021). 520 $aComo ya es tradicional en el mes de febrero, se realizó el día de campo de arroz para la zona norte. En este día de campo virtual se presentaron avances en dos líneas de investigación: mejoramiento genético y manejo del cultivo en relación a resistencia a herbicidas y manejo del riego. Al igual que en años anteriores, se presentaron resultados de la Red Nacional de Evaluación de cultivares INIA - INASE. El 4 de marzo tuvo lugar el Dia de campo de arroz para la zafra 20-21 correspondiente a la Unidad Experimental Paso de la Laguna. El objetivo de esta actividad fue que los participantes adquieran un panorama de los trabajos de investigación que INIA viene desarrollando, permitiendo integrar esta información con lo que se está haciendo a nivel productivo y generar así nuevos elementos para analizar los resultados de la investigación. 650 $aARROZ 700 1 $aINIA TREINTA Y TRES 700 1 $aINIA TACUAREMBÓ 773 $tRevista INIA Uruguay, 2021, no.64, p.103.
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